At EpiVax we use MHC binding assays to confirm that the peptides identified by the EpiMatrix System are true HLA ligands. Our binding assays utilize recombinant, soluble MHC molecules and time resolved flourescence for the highest sensitivity and the lowest background available on the market. We can perform HLA binding assays for the most common Class I and Class II alleles.
We are experienced with many cell based assays including several assays based on either ELISA or ELISpot technologies. These assays are particularly useful for monitoring T cell activation and proliferation. Cell mediated and antibody directed cytotoxicity assays can be used to assess the function of epitopes, vaccine constructs, and therapeutic proteins.
